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. 2020 Nov 24;76(Pt 12):1244–1255. doi: 10.1107/S2059798320013753

Figure 2.

Figure 2

Structural analysis of CSF-1RD1–D3 derived from HEK293 GD. (a) Comparison of HEK293T and HEK293 GD-derived CSF-1RD1–D3; the inset shows reduced SDS–PAGE analysis of the purified proteins. (b) Van der Waals contacts between GD-type glycans on Asn275. (c) Crystal-packing contacts between symmetry-related glycans. The inset shows a schematic representation of the glycan. (d) The GD-type glycan on Asn73 is α-1,6-fucosylated. The 2mF o − DF c electron-density map is shown as a gray mesh. Residual positive and negative mF o − DF c electron-density maps (contoured at ±3σ) are shown in green and red, respectively.