. 2020 Nov 24;76(Pt 12):1244–1255. doi: 10.1107/S2059798320013753

Table 1. Crystallization conditions for DSCAM_Ig7–Ig9 for the wild-type and GD-derived proteins.

	HEK293T	HEK293 GlycoDelete
Protein sample	5 mg ml⁻¹ in 20 mM HEPES pH 7.4, 100 mM NaCl, 1 mM DTT	7.5 mg ml⁻¹ in 20 mM HEPES pH 7.4, 100 mM NaCl, 1 mM DTT

Initial crystal
Screen	The PEGs II Suite	The PEGs II Suite
Condition	H9: 0.05 M magnesium acetate, 10%(w/v) PEG 8000, 0.1 M sodium acetate	H8: 0.2 M calcium acetate, 10%(w/v) PEG 8000. 0.1 M HEPES pH 7.5
Protein sample	7 mg ml⁻¹ in 20 mM HEPES pH 7.4, 100 mM NaCl, 1 mM DTT	7 mg ml⁻¹ in 20 mM HEPES pH 7.4, 100 mM NaCl, 1 mM DTT
Optimized condition	0.05 M magnesium acetate, 16%(w/v) PEG 8000	0.2 M calcium acetate, 10%(w/v) PEG 8000, 0.1 M HEPES pH 7.5, 3%(v/v) glycerol

Optimized crystal mounted in a cryo-loop
Diffraction pattern
Diffraction limit (Å)	∼8	1.85

Table 1. Crystallization conditions for DSCAMIg7–Ig9 for the wild-type and GD-derived proteins.