Table 2. Crystallographic data-collection and refinement statistics.
Values in parentheses are for the highest resolution shell.
| DSCAMIg7–Ig9 | IL-12B | CSF-1–CSF-1RD1–D3 | |
|---|---|---|---|
| Wavelength (Å) | 0.976 | 1.033 | 0.976 |
| Resolution range (Å) | 84.81–1.85 (1.89–1.85) | 67.41–2.40 (2.49–2.40) | 49.71–2.80 (2.97–2.80) |
| Space group | C2 | I41 | I41 |
| a, b, c (Å) | 78.6, 71.4, 92.2 | 85.88, 85.88, 107.67 | 143.00, 143.00, 138.32 |
| α, β, γ (°) | 90, 113.1, 90 | 90, 90, 90 | 90, 90, 90 |
| Total reflections | 148955 (9215) | 123592 (9760) | 235463 (37046) |
| Unique reflections | 39662 (2418) | 15233 (1486) | 34125 (5448) |
| Multiplicity | 3.8 (3.8) | 8.11 (6.6) | 6.9 (6.8) |
| Completeness (%) | 99.0 (97.4) | 99.7 (97.4) | 99.8 (99.2) |
| Mean I/σ(I) | 12.8 (1.6) | 12.2 (0.8) | 20.7 (2.3) |
| Wilson B factor (Å2) | 43.1 | 60.25 | 82.3 |
| R merge | 0.044 (0.874) | 0.117 (1.564) | 0.059 (0.864) |
| R meas | 0.051 (1.011) | 0.125 (1.698) | 0.064 (0.815) |
| R p.i.m. | 0.026 (0.502) | 0.044 (0.649) | 0.024 (0.351) |
| CC1/2 | 1.0 (0.7) | 0.998 (0.487) | 0.999 (0.74) |
| Reflections used in refinement | 37658 (2753) | 15215 (1480) | 34116 (3318) |
| Reflections used for R free | 2001 (153) | 1511 (144) | 1706 (165) |
| R work | 0.189 (0.289) | 0.229 (0.365) | 0.223 (0.409) |
| R free | 0.217 (0.303) | 0.265(0.437) | 0.261 (0.465) |
| No. of non-H atoms | |||
| Total | 2564 | 2412 | 6657 |
| Macromolecules | 2281 | 2306 | 6370 |
| Ligands | 116 | 73 | 287 |
| Solvent | 167 | 33 | n.a. |
| No. of protein residues | 292 | 295 | 833 |
| R.m.s.d., bond lengths (Å) | 0.01 | 0.005 | 0.006 |
| R.m.s.d., angles (°) | 1.80 | 1.04 | 1.094 |
| Ramachandran favored (%) | 96.9 | 95.79 | 95.6 |
| Ramachandran allowed (%) | 3.1 | 4.21 | 4.4 |
| Ramachandran outliers (%) | 0.0 | 0.00 | 0.0 |
| Average B factor (Å2) | |||
| Overall | 36.9 | 66.8 | 100.3 |
| Macromolecules | 33.2 | 66.4 | 97.4 |
| Ligands | 74.8 | 85.7 | 164.6 |
| Solvent | 60.4 | 56.0 | n.a. |
| No. of TLS groups | 4 | n.a. | 18 |
| PDB code | 6zr7 | 6sff | 4wrl |