Fig. 2.

Anti-tumorigenic activities on PDAC cells are unique to the M0 subset of macrophages. a BrdU proliferation assay of PANC-1 and MIA PaCa-2 cells in RPMI-1640 (negative-control) or conditioned media from different macrophage subtypes at t = 48 h. On the figure shown is the mean ± SEM (n = 4); One-way ANOVA was used to assess statistical significance. b Annexin V-FITC positive cells in negative-control (RPMI-1640), conditioned media from different macrophage subtypes, or Gemcitabine (as positive-control) treated PANC-1 and MIA PaCa-2 cells at t = 48 h. Plotted is the Geometric Mean Fluorescence Intensity (gMFI) on the FITC channel (Annexin V density). On the figure shown is the mean ± SEM (n = 3); One-way ANOVA was used to assess statistical significance