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. 2020 Dec 2;20:1183. doi: 10.1186/s12885-020-07697-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Infliximab inhibits the cytotoxic activity of M0 macrophages. a BrdU proliferation assay of PANC-1 and MIA PaCa-2 cells in RPMI-1640 (negative-control) or M0-CM with 25 μg/ml Infliximab where indicated. On the figure shown is the mean ± SEM (n = 4); One-way ANOVA was used to assess statistical significance. b Annexin V-FITC positive cells in control, M0-CM with or without 25 μg/ml Infliximab where indicated, or Gemcitabine (as positive control) treated PANC-1 and MIA PaCa-2 cells at t = 48 h. Plotted is the Geometric Mean Fluorescent Intensity (gMFI) on the FITC channel (Annexin V density). On the figure shown is the mean ± SEM (n = 3); One-way ANOVA was used to assess the significance. c Annexin V-FITC positive cells in control, M0 M-CSF, or M0 GM-CSF with or without 25 μg/ml Infliximab where indicated, or Gemcitabine (as positive-control) treated PANC-1 and MIA PaCa-2 cells at t = 48 h. Plotted is the Geometric Mean Fluorescent Intensity (gMFI) on the FITC channel (Annexin V density). On the figure shown is the mean ± SEM (n = 3); One-way ANOVA was used to assess the significance