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. 2020 Dec 1;18:177. doi: 10.1186/s12951-020-00734-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Biodistribution of silk spheres in a Her2(+) model of metastatic breast cancer. a Schematic representation of the treatment course. b Biodistribution of the control MS1 and H2.1MS1 spheres in the lungs on the 17th day after the first intravenous injection. The lungs were excised and imaged. Signal detection was performed by using the IVIS Spectrum at excitation/emission wavelengths of 640/680 nm. c Biodistribution of ATTO647N-labeled MS1 and H2.1MS1 spheres over time. Whole-body IVIS fluorescent images were obtained using the IVIS Spectrum