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. 2020 Dec 2;21:856. doi: 10.1186/s12864-020-07233-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Mouse strain edit sites. The methylation patterns of three CRISPR-edited animal strains were evaluated in this study, describe in detail in Table 1. Strain HDR1 (a) contains an 11-kb insert and was recombinantly inserted into the Rxfp3 locus of the mouse genome using a 1-kb upstream homology arm (UHA) and a 4-kb downstream homology arm (DHA). The protospacer adjacent motif (PAM) site was located at Chr15:11,037,280. Strain HDR2 (b) contains a 2005-bp insert and was recombinantly inserted into the Rosa26 locus of the mouse genome using a 1-kb UHA and a 1-kb DHA. The PAM site was located at Chr6:113,076,025. Strain NHEJ1 (c) contains a 48-bp duplex DNA oligonucleotide fragment incorporated into the Rosa26 locus by non-homologous end-joining. The PAM site was located at Chr6:113,076,025. For each figure, the yellow bar indicates the CGI location