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. 2020 Dec 2;21:856. doi: 10.1186/s12864-020-07233-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Edited CGIs affected by HDR show statistically significant p-values. In evaluating the methylation variance between CRISPR-edited and control mouse epigenomes, top CGIs for each comparison were determined by ranking of p-values observed and removing those CGIs that did not display greater than 50% of observed CpGs with methylation increases above 20%. Those top CGIs containing HDR edits had more significant p-values for methylation increase above biological variance (20% change) as compared to other CGIs across the mouse genome for HDR1 Animal (a) and HDR2 Animal (b). A CGI with a complete modification of its methylome displayed a significant increase of p-value over the control (a), while a CGI with only a partial modification displayed an increase of p-value over the control at a lesser significance (b). The top CGIs with methylation change within NHEJ1 Animal containing a NHEJ edit had no significant increase of p-value above biological variance, and the edited CGI was not among the top CGIs with methylation change (c). Comparison of CGIs in unedited Control 2 Animals displayed a low significance of change for the top CGIs (d)