Fig. 4.

LAD1 expression barely affects the expression of EMT markers, matrix metalloproteases, and integrins. a, b LAD1 knockdown had little effect on the expression of EMT markers in SW620 and Caco-2 cells. Expression of the E-cadherin, N-cadherin, and vimentin proteins was determined by immunoblots with specific antibodies against each protein (a) and by RT-PCR (b). c, d LAD1 depletion caused little change in the expression of matrix metalloproteases (c) and integrins (d) in SW620 and Caco-2 cells. The RNA levels of each gene were measured by RT-PCR. Note that the cropped blot and gel images are shown