Table 1.

Surface Treatments Groups Description

Groups	Surface Treatments
1. No Treatment/Control (C)	Mechanical finishing with laboratory carbide and diamond burs.
2. Pumice (Pum)	Mechanical finishing with laboratory carbide and diamond burs. Polishing using coarse, medium, and fine pumice. Washed with water and mild soap between each polishing cycle.
3. Varnish (Var)	Mechanical finishing with laboratory carbide and diamond burs. Polishing using coarse, medium, and fine pumice. Washed with water and mild soap between each polishing cycle. Application of 1 layer of varnish followed by curing cycle.
4. High Polishing (HPol)	Mechanical finishing with laboratory carbide and diamond burs. Polishing using coarse, medium, and fine pumice. Washed with water and mild soap between each polishing cycle. High polishing application using a dry rag wheel.
5. Chlorhexidine gluconate 0.12% (CHX)	Mechanical finishing with laboratory carbide and diamond burs. Polishing using coarse, medium, and fine pumice. Washed with water and mild soap between each polishing cycle. Soaked in chlorhexidine for 10 min.
6. Alcohol (Al)	Mechanical finishing with laboratory carbide and diamond burs. Polishing using coarse, medium, and fine pumice. Washed with water and mild soap between each polishing cycle. Soaked in alcohol for 10 min (leaving some alcohol behind vs drying it).
7. Steam (St)	Mechanical finishing with laboratory carbide and diamond burs. Polishing using coarse, medium, and fine pumice. Washed with water and mild soap between each polishing cycle. Disinfected using steam.