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. 2020 Dec 2;22:133. doi: 10.1186/s13058-020-01370-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Mutp53 promotes cell proliferation. a Western blot analysis of p53 and p21 levels in mouse ErbB2 mammary epithelial tumor cell lines with different p53 status. Actin is loading control. b Growth curve of mouse ErbB2 mammary epithelial tumor cell lines with different p53 status. n = 3 independent experiments per genotype (one cell line per genotype except for p53+/+ and p53 H/− where 2 different cell lines derived from different tumors and result per genotype was averaged). c Bar graph showing percent mitotic cells in mouse ErbB2 mammary epithelial tumor cell lines with different p53 status. Each bar represents the average percent of mitotic per genotype counted from at least 5 randomly selected fields at × 400 magnification (one cell line per genotype except for p53+/+ and p53 H/− where 2 different cell lines derived from different tumors and result per genotype was averaged). d Bar graph showing relative mRNA expression level of p21 in ErbB2 mammary epithelial tumor cell lines with different p53 status. n = 3 independent experiments per cell line per genotype. e Western blot analysis of p53 and p21 levels in mouse ErbB2 mammary epithelial tumor cell lines with different p53 status before and after CRISPR/Cas9 p53 deletion (p53CC9). Hsc70 is loading control. f Growth curve of mouse ErbB2 mammary epithelial tumor cell line with mutp53, before and after CRISPR/Cas9 p53 deletion (p53CC9). n = 3 independent experiments per cell line. Where applicable *p < 0.05; **p < 0.01; ***p < 0.001. Error bars represent ± SD