Fig. 3.

Mutp53 increases centrosomal aberrations and clustering. a Bar graph showing percent of cells with micronuclei before and 24 h after γ-irradiation in ErbB2 mammary epithelial tumor cell lines with different p53 status. n = 3 independent experiments per cell line per genotype (one cell line per genotype except for p53+/+ and p53 H/− where 2 different cell lines derived from different tumors and p53CC9 3 different cell lines, and result per genotype was averaged). b Immunofluorescence staining showing centrosome clustering in mitotic p53H/−;ErbB2-mouse mammary epithelium tumor cell line. Centrosomes identified by γ-tubulin staining (red) and DNA by DAPI (blue). b (A–C) Normal bipolar mitosis with one centrosome at each side. b (D–F) Bipolar mitosis showing supernumerary centrosome (≥ 3) clustering, 2 centrosomes on each side. b (G–I) Multipolar mitosis showing failure of supernumerary centrosomes to cluster. c–e Bar graphs showing percent of cells with ≥ 3 centrosomes, with centrosome clustering and with multipolar spindle, respectively, in ErbB2 mammary epithelial tumor cell lines with different p53 status. n = 3 independent experiments per genotype. f–h Bar graphs showing percent of cells with ≥ 3 centrosomes, with centrosome clustering and with multipolar spindle, respectively, in p53H/−;ErbB2 cell line before and after CRISPR/Cas9 p53 deletion (p53CC9) and in p53−/−;ErbB2 cell line. n = 3 independent experiments per genotype (for c–h, one cell line per genotype except for p53+/+ and p53 H/− where 2 different cell lines derived from different tumors and p53CC9 3 different cell lines, and result per genotype was averaged). Where applicable *p < 0.05; **p < 0.01; ***p < 0.001. Error bars represent ± SD