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. 2020 Nov 17;21(1):47. doi: 10.3892/ol.2020.12308

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Conditioned media from FGL2-OE Hepa1-6 cells increased the proportion of MDSCs in mouse BM cells. (A) Hepa1-6 cells stably transfected with LV5-FGL2 or LV5-NC lentivirus (48 h post-transfection). Light and green fluorescence microscopy images (magnification, ×4). (B) One week post LV5-FGL2 lentivirus transfection, FGL2 mRNA expression level in FGL2-OE Hepa1-6 cells was upregulated 1168 fold compared with NC Hepa1-6 cells according to reverse transcription quantitative PCR experiment. (C) FGL2 protein expression was increased in FGL2-OE Hepa1-6 cells compared with NC Hepa1-6 cells according to western blotting experiment. (D) FGL2 protein level was significantly higher in FGL2-OE Hepa1-6 cell culture supernatants compared with NC Hepa1-6 cell supernatants according to ELISA assay. (E) BM cells extracted from C57BL/6 mice femurs were cultured for 24–48 h using conditioned media from FGL2-OE Hepa1-6 cell, NC Hepa1-6 cell or conventional medium. (F and G) Proportion of CD11b⁺/Gr-1⁺ cells in BM cells cultured in FGL2-OE Hepa1-6 conditioned media for 24 and 48 h was higher compared with BM cells cultured in NC Hepa1-6 conditioned media (n=8). ***P<0.001. BM, bone marrow; FGL2, fibrinogen-like protein 2; MDSCs, myeloid-derived suppressor cells; NC, negative control; OE, overexpressing.