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SDS–PAGE of supernatants using 12% acrylamide gel of (a) C-coll hydrogel in Tris buffer, pH 7.4 (CB); (b) D-coll hydrogel in Tris buffer, pH 7.4 (DB); (c) C-coll hydrogel in collagenase solution in Tris buffer, pH 7.4 (CC) and (d) D-coll hydrogel in collagenase solution in Tris buffer, pH 7.4 (DC). The lane of protein marker is labelled as M and shows the corresponding molecular weights. The collagen bands of α chains, β chains and high molecular weight hydrolyzed peptides (HHP).
