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Sensitivity analysis of the singleplex RPA assay using synthetic molecular DNA standard. The assay showed a sensitivity of 10 copies/reaction in T. pallidum and H. ducreyi. RPA reaction tubes were removed and quick vortexed/mixed four minutes after state of amplification and hence no florescence recorded. Sensitivity test for T. pallidum (a) and H. ducreyi (b). “Neg” is negative control or no template control (NTC).
