Table 1.

RPA Primer and Probe for T. pallidum and H. ducreyi Detection.

Name	Sequence	Amplicon Size
TP_RPA_FP3	GTAACACTAATGTCGAGACTGAAAAGGAGTGC	144 bp
TP_RPA_RP2	GAATGATGAGACGCTCACACTTGTTATGC
TP_RPA_P1	GTATCTGCATCTGCTGTGCAGGATCCGGCA (BHQ2-dT) (X-dSpacer) (ROX-dT)GTCCAAGCTGTCATG-PH
Hd_RPA_FP3	TACTCAGGCAACGGATACCCAACATGCA	169 bp
Hd_RPA_RP1	GAGGTAAATCAGGCTGTTACAGGTCATTTA
Hd_RPA_P1	TACGCCTAAATCGTTAACTGCGGGATTAGG (BHQ2-dT) (X-dSpacer) (FAM-dT)AGATGGCCATGGTAG-PH

FP: Forward primer, RP: Reverse primer, P: probe, FAM-dT and ROX-dT: thymidine nucleotide carrying Fluorochrome; dSpacer: tetrahydrofuran basic site mimic, BHQ2-dT: thymidine nucleotide carrying Black Hole quencher, PH: 3′ phosphate group.