Skip to main content
. 2020 Nov 19;45(1):95–106. doi: 10.3892/or.2020.7862

Figure 2.

Figure 2.

ERBB2 overexpression in cervical cancer cell lines stimulates viability, invasion and sphere-formation. (A) Confirmation of ERBB2 KD in siERBB2 cells and OE in ERBB2 vec cells via WB. GAPDH was used as the loading control. (B) Invasion of siERBB2 vs. siCtrl cells via Transwell assay. (C) Invasion of ERBB2 vec vs. Ctrl vec cells via Transwell assay. (D) Cellular viability of siERBB2, siCtrl, ERBB2 vec and Ctrl vec cells quantified using a Cell Counting Kit-8. (E) Sphere-formation of siERBB2 vs. siCtrl cells. (F) Sphere-formation of ERBB2 vec vs. Ctrl vec cells. (G) Analysis of metastasis-associated and cancer stem cell biomarkers mRNA and protein expression in siERBB2, siCtrl, ERBB2 vec and Ctrl vec cells via RT-qPCR and WB, respectively. GAPDH was used as the RT-qPCR housekeeping control and WB loading control. Data are expressed as the mean ± SEM (n=3). **P<0.01 vs. siCtrl or Ctrl vec analyzed via unpaired Student's t-test. KD, knockdown; OE, overexpression; WB, western blotting; RT-qPCR, reverse transcription-quantitative PCR; si, small interfering; Ctrl, control; vec, vector; ERBB2, Erb-B2 Receptor Tyrosine Kinase 2.