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. 2020 Oct 26;45(1):202–216. doi: 10.3892/or.2020.7825

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — GAS5 acts as a molecular sponge of miR-34a in CRC HT29 and SW480 cells. (A) Data from DIANA Tools showed a common binding site for GAS5 and miR-34a. (B) Luciferase reporter assay showed a reduction in luciferase activity of wild-type (WT) GAS5 3′ UTR following transfection with miR-34a mimic in CRC cells. (C) RT-qPCR analysis of expression of GAS5 in CRC cells after pcDNA3.1-GAS5 transfection. (D) RT-qPCR analysis of expression of miR-34a in CRC cells after pcDNA3.1-GAS5 transfection. (E) SIRT1 protein expression was examined by western blotting. ***P<0.001 indicates a significant difference vs. the normal control group. CRC, colorectal cancer; GAS5, growth arrest specific 5; miR/miRNA, microRNA; SIRT1, sirtuin 1.