Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used for RT-qPCR.
| Primer name | Primer sequence: 5′-3′ |
|---|---|
| Forward GAS5 | CTTGCCTGGACCAGCTTAAT |
| Reverse GAS5 | CAAGCCGACTCTCCATACCT |
| Forward mTOR | TCCGAGAGATGAGTCAAGAGG |
| Reverse mTOR | CACCTTCCACTCCTATGAGGC |
| Forward SIRT1 | CCCAGAACATAGACACGCTGGA |
| Reverse SIRT1 | ATCAGCTGGGCACCTAGGACA |
| Forward β-actin | ATGTTGAGACCTTCAACACC |
| Reverse β-actin | AGGTAGTCAGTCAGGTCCCGGCC |
| Forward miR-34a | TGGTGTCGTGGAGTCG |
| Reverse miR-34a | GGCATCTCTCGCTTCATCTT |
| Forward U6 | CTCGCTTCGGCAGCACA |
| Reverse U6 | AACGCTTCACGAATTTGCGT |
