Table II.
Antibodies used for western blot analysis.
| Antibody | Product/cat. no. (manufacturer) | Species | Dilution |
|---|---|---|---|
| mTOR | sc-517464 (Santa Cruz Biotechnology, Inc., USA) | Mouse | 1:200 |
| p-mTOR | sc-293089 (Santa Cruz Biotechnology, Inc., USA) | Mouse | 1:200 |
| SIRT1 | sc-135792 (Santa Cruz Biotechnology, Inc., USA) | Mouse | 1:500 |
| LC3 | sc-28266 (Santa Cruz Biotechnology, Inc., USA) | Rabbit | 1:500 |
| Beclin1 | sc-11427 (Santa Cruz Biotechnology, Inc., USA) | Rabbit | 1:500 |
| Bax | sc-526 (Santa Cruz Biotechnology, Inc., USA) | Mouse | 1:200 |
| Bcl-2 | sc-7382 (Santa Cruz Biotechnology, Inc., USA) | Mouse | 1:200 |
| MMP2 | sc-13594 (Santa Cruz Biotechnology, Inc., USA) | Mouse | 1:500 |
| MMP9 | sc-393859 (Santa Cruz Biotechnology, Inc., USA) | Mouse | 1:500 |
| β-actin | sc-69879 (Santa Cruz Biotechnology, Inc., USA) | Mouse | 1:500 |
| Anti-rabbit IgG, Alexa Fluor 680 | A10043 (Thermo Fisher Scientific, Inc., USA) | Donkey | 1:5,000 |
| Anti-mouse IgG, Alexa Fluor 680 | A32788 (Thermo Fisher Scientific, Inc., USA) | Donkey | 1:5,000 |