Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 19;45(1):83–94. doi: 10.3892/or.2020.7859

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 1. — PDGF-BB affects cell proliferation, apoptosis resistance and migration of pancreatic cancer cells. (A) MTT assay of cell proliferation after BxPC-3 cells were incubated with increasing concentrations of PDGF-BB (0–100 ng/ml) for 24 h. PBS was used as a control. (B) Flow cytometric analysis of apoptosis in BxPC-3 cells exposed to 100 ng/ml PDGF-BB or PBS and cultured in suspension for 48 h. (C) Percentage of apoptotic cells analyzed in three independent experiments. (D) Wound-healing assays of BxPC-3 cells in the presence or absence of 100 ng/ml PDGF-BB. Wounds were allowed to heal for 24 h and imaged under a microscope. (E) Wound-healing area analyzed in three independent experiments. (F) Transwell assay of cell invasion. BxPC-3 cells in the presence or absence of 100 ng/ml PDGF-BB were induced to invade through Matrigel for 24 h. (G) Number of invading cells in 6 wells was analyzed in three independent experiments. Results are presented as the mean ± SD (n≥3). *P<0.05 and **P<0.01 vs. mock/PBS. PDGF-BB, platelet-derived growth factor-BB.