Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 19;45(1):83–94. doi: 10.3892/or.2020.7859

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 2. — PDGF-BB affects YAP dephosphorylation, subcellular localization and downstream gene expression. (A) Western blot analysis of YAP, MST1/2 and LATS1 expression and their phosphorylation levels in BxPC-3 cells exposed to 100 ng/ml PDGF-BB or PBS. (B) Immunofluorescence staining exhibiting the subcellular localization of YAP protein after treatment of BxPC-3 cells with 100 ng/ml PDGF-BB for 24 h. (C) BxPC-3 cells were transfected with the 8×GTIIC-luc reporter and Renilla-luc plasmids and cultured under increasing concentrations of PDGF-BB (0–100 ng/ml) for 24 h. Relative firefly/Renilla luciferase activity was measured. (D) Reverse transcription-quantitative PCR analysis of the expression levels of YAP target genes (MYC and MCL-1) in BxPC-3 cells treated with PDGF-BB (0–100 ng/ml) or PBS. Relative mRNA levels were normalized to GAPDH. (E) Western blot analysis of E-cadherin and N-cadherin expression in BxPC-3 cells after PDGF-BB treatment. (F) mRNA levels of E-cadherin and N-cadherin relative to GAPDH in the mock and PDGF groups. Data are presented as the mean ± SD (n=3). *P<0.05 and **P<0.01 vs. mock or 0 ng/ml. PDGF-BB, platelet-derived growth factor-BB; YAP, Yes-associated protein; p, phosphorylated; MST1, Macrophage Stimulating 1; LATS1, Large Tumor Suppressor Kinase 1.