Figure 4.

miR-637 suppresses proliferation, migration and invasion partially via HDAC4 modulation in osteosarcoma cells. (A) HDAC4 mRNA expression in HOS and U2OS cells was measured by reverse transcription-quantitative PCR. (B) HDAC4 protein expression was investigated by western blotting. Metastatic and proliferation abilities of (C) HOS and (D) U2OS cells were determined by Transwell and Cell Counting Kit 8 assays. Magnification, ×20; scale bar, 200 µm. A luciferase assay was used to demonstrate the targeted binding effect between miR-637 and HDAC4 in (E) HOS and (F) U2OS cells. **P<0.01. All data are presented as the mean ± SD of three independent experiments. HDAC4, histone deacetylase 4; LUC, luciferase; miR-637, microRNA-637; MUT, mutant; NC, negative control; n.s, not significant (P>0.05); OD, optical density; oe, overexpression; siRNA, small interfering RNA; WT, wild-type.