Table I.
Primers/oligonucleotides | Sequence (5′-3′) |
---|---|
circHIPK3 forward | TATGTTGGTGGATCCTGTTCGGCA |
circHIPK3 reverse | TGGTGGGTAGACCAAGACTTGTGA |
HIPK3 forward | TGGAGACTGGGGGAAGATGA |
HIPK3 reverse | CACACTAACTGGCTGAGGGG |
HDAC4 forward | TCAGACATCTTTGGGAAGGG |
HDAC4 reverse | CAACCTCCATCTTGCCTTGT |
GAPDH forward | GTCAAGGCTGAGAACGGGAA |
GAPDH reverse | AAATGAGCCCCAGCCTTCTC |
miR-637 forward | ACUGGGGGCUUUCGGGCUCUGCGU |
miR-637 reverse | ACGCAGAGCCCGAAAGCCCCCAGU |
U6 forward primer | CTCGCTTCGGCAGCACA |
U6 reverse primer | AACGCTTCACGAATTTGCGT |
sicircHIPK3_1 sense | UGGCCUCACAAGUCUUGGU |
sicircHIPK3_1 antisense | ACCAAGACUUGUGAGGCCAUA |
sicircHIPK3_2 sense | GGUACUACAGGUAUGGCCUTT |
sicircHIPK3_2 antisense | AGGCCAUACCUGUAGUACCGA |
siHDAC4 sense | GAAAAGGUUUUACAGCAAATT |
siHDAC4 antisense | UUUGCUGUAAAACCUUUUCTG |
miR-637 mimic | ACUGGGGGCUUUCGGGCUCUGCGU |
miR-637 inhibitor | ACGCAGAGCCCGAAAGCCCCCAGU |
circ, circular RNA; HDAC4, histone deacetylase 4; HIPK3, homeodomain interacting protein kinase 3; miR-637, microRNA-637; si, small interfering RNA.