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. Author manuscript; available in PMC: 2021 Apr 2.
Published in final edited form as: Sci Immunol. 2020 Oct 2;5(52):eabd2876. doi: 10.1126/sciimmunol.abd2876

Fig. 6. Pyroptosis mediator Gasdermin is not required for IL-18-induced cell death or its protection against mRV infection.

Fig. 6

(A-D) Gasdermin-deficient, or WT, mice were administered PBS or IL-18 (2 μg) three days post-mRV inoculation. Mice were euthanized 6 h later and jejunums were analyzed. (A and B) IEC were analyzed by SDS-PAGE immunoblotting for detection of gasdermin-D, cleaved gasdermin D and cleaved caspase 3, respectively. (C) Cell death by TUNEL, counterstained with DAPI. (D) Quantitation of TUNEL-positive cells at villus tip region based on visual counts. Experiments included 5 mice per condition. Data in panel G was based on assay 6–8 villi per mouse, which are indicated by data points **** indicates P < 0.0001 by Student’s t test. (E) Gasdermin-deficient mice were administered PBS or IL-18 (2 μg) via intraperitoneal injection, 2 hours prior to, or 2, 4, 6 or 8 days after (indicated by arrows), oral inoculation with mRV. Fecal RV levels were measured over time by ELISA. . Data are mean ± SEM, n=5 * indicates significantly different from control by two-way ANOVA, P < 0.0001.