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. Author manuscript; available in PMC: 2020 Dec 2.
Published in final edited form as: Cell Rep. 2020 Nov 17;33(7):108401. doi: 10.1016/j.celrep.2020.108401

Figure 4. UV Light Induces Novel Tandem Mutations in Yeast.

Figure 4.

(A) Spectrum of tandem mutations derived from genome sequencing of WT yeast following repeated UV exposure (9 or 15 doses).

(B) UV-induced tandem substitutions are elevated in repair-deficient rad16Δ mutant cells. Mutations per sequenced isolate for 15 dose experiments are plotted. Significant differences in the number of mutations in each mutation class per isolate in WT relative to the rad16Δ mutant strain was determined using a t test with the Holm-Sidak correction for multiple hypothesis testing. **p < 0.001; *p < 0.01.

(C and D) UV-induced tandem substitutions are elevated on the NTS of yeast genes in WT and rad16Δ mutant cells. Statistical significance was determined using the chi-square test and Bonferroni correction for multiple hypothesis testing. **p < 0.001; *p < 0.05.

(E) Sequence logo representation of DNA flanking all AC>NN tandem substitutions (e.g., AC>TT, AC>CT, etc.) in yeast. Logo was generated using weblogo (Crooks et al., 2004).