KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| E. coli: BL21 pLysS | Thermo Fisher Scientific | Cat#C606003 |
| E. coli: T7 Express | New England BioLabs | Cat#C3010I |
| E. coli: DH5α | Thermo Fisher Scientific | Cat# 18258012 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| T4 polynucleotide kinase | New England BioLabs | Cat# M0201L |
| T4 DNA ligase | New England BioLabs | Cat#M0202T |
| NEBNext End Repair Module | New England BioLabs | Cat#E6050L |
| NEBNext dA-Tailing Module | New England BioLabs | Cat#E6053L |
| Terminal Transferase | New England BioLabs | Cat#M0315L |
| UVDE | This Study | NA |
| CPD photolyase | This Study | NA |
| shrimp alkaline phosphatase | Affymetrix | Cat#AF78390500 |
| NEBNext® Quick Ligation Module | New England BioLabs | Cat#E6056L |
| Streptavidin beads | Thermo Fisher Scientific | Cat#11205D |
| Critical Commercial Assays | ||
| KAPA DNA HyperPrep | Roche Scientific | Cat# 07962363001 |
| Deposited Data | ||
| Saccer3 yeast reference genome | Saccharomyces Genome Database | https://hgdownload.soe.ucsc.edu/goldenPath/sacCer3/bigZips/sacCer3.fa.gz |
| Yeast transcription start and end sites | (Park et al., 2014) | NA |
| Melanoma Mutation Data | International Cancer Genome Consortium | https://dcc.icgc.org/api/v1/download?fn=/release_20/Projects/MELA-AU/simple_somatic_mutation.open.MELA-AU.tsv.gz |
| Cutaneous squamous cell carcinoma mutation data | (Zheng et al., 2014) | NA |
| RPKM normalized RNA-seq data for melanocytes (E059) | Roadmap Epigenome | https://egg2.wustl.edu/roadmap/data/byDataType/rna/expression/ |
| RPKM normalized RNA-seq data for keratinocytes (E057) | Roadmap Epigenome | https://egg2.wustl.edu/roadmap/data/byDataType/rna/expression/ |
| GENCODE (version 10, hg19) | (Harrow et al., 2012) | NA |
| Mutational status of the BRAF gene | COSMIC Database | https://cancer.sanger.ac.uk/cosmic/gene/analysis?ln=BRAF |
| Mutational status of the NRAS gene | COSMIC Database | https://cancer.sanger.ac.uk/cosmic/gene/analysis?ln=NRAS |
| UVDE-seq | This Study | GEO: GSE144679 |
| Yeast whole genome sequencing | This Study | SRA: PRJNA605561 |
| List of UV-induced mutations in yeast | This Study | Data S1 |
| Experimental Models: Organisms/Strains | ||
| S. cerevisiae: BY4741 (Wild Type) MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Dharmacon, Inc | Cat#YSC1048 |
| S. cerevisiae: BY4742 (Wild Type): MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | ATCC | Cat#201389 |
| S. cerevisiae: MP072 (rad16Δ): MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 trp1::HIS3 rad16::TRP1 | (Mao et al., 2017) | NA |
| S. cerevisiae: MP071 (rad26Δ): MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 trp1::HIS3 rad26::TRP1 | (Mao et al., 2017) | NA |
| S. cerevisiae: YML153 (rad16Δ): MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 rad16::LEU2 | This Study | NA |
| S. cerevisiae: YML150 (rad26Δ): MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 rad26::LEU2 | This Study | NA |
| S. cerevisiae: BY4743 (Wild Type diploid): MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0 | Dharmacon, Inc | Cat#YSC1050 |
| S. cerevisiae: YML155 (rad16Δ diploid): MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0 TRP1/trp1::HIS3 rad16::TRP1/rad16::LEU2 | This Study | NA |
| S. cerevisiae: YML152 (rad26Δ diploid): MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0 TRP1/trp1::HIS3 rad26::TRP1/rad26::LEU2 | This Study | NA |
| S. cerevisiae: ySR185 (WT diploid): MATa/α his7-2/his7-2 ura3Δ/ura3Δ can1Δ/can1Δ ade2Δ/ade2Δ leu2-3,112/leu2-3,112 trp1-289/trp1-289 lys2::ADE2-URA3-CAN1/lys2::ADE2-URA3-CAN1 | This Study | NA |
| S. cerevisiae: YHSM1 (trp5 E50V): MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 trp5-E50V | This Study | NA |
| S. cerevisiae: ySR128 (Wild Type): MATα his7-2 ura3Δ can1Δ ade2Δ leu2-3,112 trp1-289 lys2::ADE2-URA3-CAN1 | (Roberts et al., 2012) | NA |
| S. cerevisiae: DM01 (ura3 K93V): MATα his7-2 ura3Δ can1Δ ade2Δ leu2-3,112 trp1-289 lys2::ADE2-ura3-K93V-CAN1 | This Study | NA |
| S. cerevisiae: DM02 (ura3 K93V): MATα his7-2 ura3Δ can1Δ ade2Δ leu2-3,112 trp1-289 lys2::ADE2-ura3-K93V-CAN1 | This Study | NA |
| S. cerevisiae: DM03 (ura3 K93V diploid): MATa/α his7-2/his7-2 ura3Δ/ura3Δ can1Δ/can1Δ ade2Δ/ade2Δ leu2-3,112/leu2-3,112 trp1-289/trp1-289 lys2::ADE2-URA3-CAN1/lys2::ADE2-ura3-K93V-CAN1 | This Study | NA |
| S. cerevisiae: DM04 (ura3 K93V diploid): MATa/α his7-2/his7-2 ura3Δ/ura3Δ can1Δ/can1Δ ade2Δ/ade2Δ leu2-3,112/leu2-3,112 trp1-289/trp1-289 lys2::ADE2-URA3-CAN1/lys2::ADE2- ura3-K93V-CAN1 | This Study | NA |
| Oligonucleotides | ||
| Primers: see Table S3 | This Study | NA |
| Recombinant DNA | ||
| T. thermophiles UVDE cDNA | UniProtKB | ID#Q746K1 |
| E. coli CPD photolyase; gene name phrB | UniProtKB | ID#P00914 |
| pETHT | (Brady et al., 1996) | NA |
| pET16b | MilleporeSigma | Cat#69662 |
| pAB001 | This Study | NA |
| pET16b-CPDphotolyase | This Study | NA |
| YEpHO | (Murray and Szostak, 1983) | NA |
| pRS405 | ATCC | Cat#87516 |
| Software and Algorithms | ||
| CLC genomics workbench version 7.5 | QIAGEN | Cat#832001 |
| ImageQuant TL | GE Biosciences | Cat#29000737 |
| bowtie2 | (Langmead and Salzberg, 2012) | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml |
| GraphPad Prism 6 | GraphPad Software | https://www.graphpad.com/ |