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. Author manuscript; available in PMC: 2021 Jul 2.
Published in final edited form as: ACS Catal. 2020 Jun 4;10(13):7112–7116. doi: 10.1021/acscatal.0c01888

Figure 1.

Figure 1.

Directed evolution of RmaNOD for production of cyclopropylboronate 3. All reactions were performed with whole E. coli resuspended in 20 mM MOPS buffer (pH = 7) to OD600=30, 20 mM 1, 40 mM 2, anaerobic conditions, 5% EtOH cosolvent. Experimental details are provided in the Supporting Information, Materials and Methods section. (a) Diastereomeric ratio (%cis) and total turnover number (TTN) of the evolutionary lineage toward production of cis-3. (b) Diastereomeric ratio (%trans) and TTN of the evolutionary lineage of trans-3 production.