Fig. 2.

Evaluation of segmentation accuracy by Imaris, Icy and Phagosight relative to a ground truth. The number of segmented objects by Imaris (purple), Icy (green) and Phagosight (cyan) were compared to a manually defined ground truth (dark blue) from datasets 1–3 (A). The volumes of the 10 segmented objects from dataset 1 were compared relative to a ground truth by plotting average values with standard deviation at 5, 9.5 and 14.5 h post injury (hpi) for each method (B). The effectiveness of each method for detecting the 10 cells at three time points (representing 30 data points) were scored as good (blue), none (red) or false (green) for each dataset (C–E). The volumes of the selected 30 objects segmented by each method were compared by star plots in which the outputs of the three programs were normalised to the ground truth (blue) for each dataset (F–H).