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. 2020 Nov 24;13(11):dmm044354. doi: 10.1242/dmm.044354

Fig. 4.

Fig. 4.

Defects in T-tubule and triad formation. T-tubule and BIN1 localization finish to mature around birth, and BIN1 is still partially longitudinal in newborn mice. (A) BIN1 detected with a pan-isoform antibody localizes on intracellular longitudinal tubules in newborn isolated myofibers, a pattern lost in Bin1ex20−/− mice. Signal is shown in black over white. (B) DHPR aggregation in Bin1ex20−/− isolated myofibers from newborn mice. (C) MTM1 localization was disrupted in Bin1ex20−/− isolated myofibers. (D) Primary muscle cells differentiated into myotubes in culture and with FM4-64 staining of sarcolemma-connected membrane tubules; Bin1ex20−/− myotubes lack the dense tubules network. Scale bars: 5 µm.