Fig. 1.

puf-8; plp-1 double mutant germ lines do not produce oocytes. Germ lines extruded from 1-day-old adults and stained with DAPI are shown. Approximate boundaries of different developmental stages are indicated by vertical lines and labeled above the wild-type germ line. Although oocyte nuclei can be seen in wild-type and the two single mutant germ lines, they are absent in the puf-8; plp-1 double mutant. The double mutant germ lines are relatively shorter, and the chromatin morphologies characteristic of the different developmental stages are not discernible in them. In some of these double mutant germ lines, proliferating germ cells are seen in the proximal region. Scale bar: 20 µm.