Fig. 1.

The effect of TGFβ1 on ATG protein levels and LC3B lipidation in A549 cells. (A) A549 cells were treated with 250 pM TGFβ1 for 24 h. Cells were lysed and subjected to SDS-PAGE and immunoblotting anti-ATG3, anti-ATG5, anti-ATG7, anti-ATG9, anti-ATG12, anti-ATG12-ATG5 complex, anti-ATG16L1, anti-BECN1, anti-ULK1, anti-LC3B, anti-P-Smad2, anti-Smad2 and anti-GAPDH (loading control) antibodies. (B) The steady state levels of ATG3, ATG5, ATG7, ATG9, ATG12, ATG12-ATG5, ATG16L1, BECN1, ULK1, and LC3B were quantitated using QuantityOne software and graphed (n=3±s.e.m.). Significance is indicated as *=P<0.05, **P<0.01 and ****=P<0.0001.