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Metabolite flux analysis of 769-P RCC cells with or without L2HGDH, incubated with [U-13C] glucose. 769-P RCC cells were stably transduced with control vector or L2HGDH. Cells were then treated with [U-13C] glucose for 24 h followed by metabolite extraction and LC-MS profiling. Values represent total 13C label incorporation into the indicated metabolite. Graph depicts two-tailed unpaired Student's t-test results and data are means±s.e.m.
