A) Top, air nozzle tip is located approximately 4 cm above whiskers. Bottom, three whiskers analyzed in B and S1 Fig. Scale bars, 1 cm. B) Example movement traces of whisker 1 indicated in A at 3 different air puff strengths; 1, 5, and 9 psi. Black bars at the bottom indicate air puff (500 ms). C) Average movement (area under the curve in B) across 50 trials for whisker 1 at 3 different air puff strengths. D) Normalized whisker motion energy (arbitrary unit, see methods) before and after air puff stimulus in an example awake head fixed animal. Black bar at the top indicates air puff. E) Whisker motion energy averaged across all trials for each animal, N = 3 animals. Black bar indicates air puff.