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B cell and B cell subset PBMCs stained with CD19–APC, CD27-FITC, and CD38-PE gated on lymphocyte (R1), total B cells (R2, CD19+), naïve B cells (R3, CD19+CD27-), memory B cells (R4, CD19+CD27+), and then plasma B cells (R5, CD19+CD27hiCD38hi).
