Fig 4. CFTR expression in human and mouse islets and in MIN6 β-cells.

A, B. Immunoblots of protein extracts obtained from purified human islets, the β-cell line MIN6 and mouse pancreas as positive control (A) and primary mouse islets (B). Bands of expected size are shown for human/mouse Cftr (C band: ~170kDa, B band: ~150kDa) detected using Cftr antibody 24–1. Actin was used as loading control. C-E. Representative original RT-PCR experiments showing Cftr transcripts of expected sizes (Table 3) amplified from total RNA obtained from primary human (n = 5 donors), mouse islets (n = 3 preparations) (C and D, respectively) and MIN6 β-cells (n = 4 preparations) (E). F. Partial sequence chromatograms of purified human CFTR amplicons, as indicated in C.