Table 1. List of primary antibodies raised against the indicated antigens are identified by their clone/catalogue number from vendors/suppliers.
| Antibody | Supplier | Dilution | Tested application | Comments |
|---|---|---|---|---|
| Cftr (CFF-432) | Cystic Fibrosis Foundation (CFF) | 1:250 | IHC/WB | Specific, tested against KO tissues, not suitable for WB |
| Cftr (CFF-217) | CFF | 1:250 | IHC/WB/IF | Specific, tested against KO tissues, not suitable for WB |
| Cftr (CFF-412) | CFF | 1:250 | IHC/WB | Specific, tested against KO tissues, not suitable for WB |
| Cftr (CFF-596) | CFF | 1:100 | WB | Not specific, not suitable for WB |
| Cftr (10B6.2) | CFF | 1:100 | WB | Specific, not suitable for WB |
| Cftr (CFF-570) | CFF | 1:100 | IHC/WB | Specific, not suitable for WB |
| Cftr (CFF-770) | CFF | 1:100 | IHC/WB | Not suitable for IHC/WB |
| Cftr (CFF-154) | CFF | 1:50 | IHC | No signal |
| Cftr (CFF-660) | CFF | 1:100 | IHC | Not specific, high background |
| Cftr (CFF-450) | CFF | 1:50 | IHC | Strong staining in islets and weak in ducts |
| Cftr (MA1-935) | Thermo Scientific | 1:400 | IHC | Stains islets and ducts |
| Cftr (Ab4067) | Abcam | 1:100 | IHC | No signal in human pancreas, weak in mouse pancreas |
| Cftr (Ab-3 L1B4) | Neomarkers | 1:50 | IHC | No signal |
| Cftr (acl-006) | Alomone | 1:250 | IF | Specific |
| Cftr (24–1) | R&D | 1:250 | WB | Specific, not suitable for IF |
| Cftr (MrPink) | CFTR Folding Consortium | 1:250 | IF | Specific |
| Cftr (3G11) | CFTR Folding Consortium | 1:250 | IF | Specific |
| Cftr (LS-B5115) | Ls-Bio | 1:200 | IF | No signal |
| Cftr (NB300-511) | Novus Biologicals | 1:100 | IF | Very weak signal |
| Cftr (20738-1-ap) | Proteintech | 1:100 | IF | No signal |
| Cftr (Mab3484) | Millipore | 1:250 | IHC/IF | Not suitable for IF |
| Insulin (273A-16) | Cell Marque | 1:500 | IF/IHC | Widely used, considered specific |
| Glucagon (K79bB10) | Abcam | 1:500 | IF/IHC | Widely used, considered specific |
| Glucagon (259A-15) | Cell Marque | 1:500 | IF/IHC | Widely used, considered specific |
| Somatostatin (ab30788) | Abcam | 1:250 | IF/IHC | Widely used, considered specific |
| Synaptophysin (ab8049) | Abcam | 1:200 | IF/IHC | Widely used, considered specific |
| PP (ab77192) | Abcam | 1:250 | IF/IHC | Widely used, considered specific |
| E-cadherin (ab6528) | Abcam | 1:100 | IF | Widely used |
| Actin (JLA20) | DHSB | 1:5000 | WB | Specific |
Dilutions and applications used are indicated together with comments regarding their specificity/reliability/suitability. Note: suitability refers to the ability of the antibody to detect low expression of the antigen in the pancreatic islet. CFTR antibodies were tested in pancreatic tissue slides and/or protein extracts, as indicated.