Table 3. List of primer-sets used in RT-PCR experiments named after the target transcript followed by numbers indicating amplicon sizes in base pairs (bp).
| Primer set | Sense | Antisense | RefSeq | Species |
|---|---|---|---|---|
| Cftr-509 | GTCATTCGACGAGTTCTAAAACAAG | CAAGAGTATATCCACAGGTATTGTCC | NM_021050 | mouse |
| Cftr-511 | CTAGTAGTCTTTATTTTACTGAGGGCC | CTAAAACGTCAGATGATCCTTCTCTAG | ||
| Cftr-567 | ATGTCGAGTCCAACCTGAATTTAG | CGGCTTGACAACTTTAAAGTCTTC | ||
| CFTR-457 | CATTCTGTTCTCAGTTTTCCTGGATTATGC | CTAAAGTCTGGCTGTAGATTTTGGAGTTCT | NM_000492 | human |
| CFTR-547 | CTCAAGAAACTGGCTTGGAAATAAGTGAAG | ATGAAGTCAAATATGGTAAGAGGCAGAAG | ||
| CFTR-583 | AGGGAGAATGATGATGAAGTACAGAGATCA | GAGAAATTACTGAAGAAGAGGCTGTCATC | ||
| CFTR-594 | CTGAATTTACATACTGCCAACTGGTTCTTG | CAAAGTTATTGAATCCCAAGACACACCATC | ||
| Krt19-544 | CCTGAAGAAGAACCATGAGGAGGA | CAGATTGTTGTAGTGGGCTTCCTG | NM_008471 | mouse |
| GAPDH-555 | GTGAAGGTCGGAGTCAACGGATTT | CACAGTCTTCTGGGTGGCAGTGAT | NM_002046 | human |
Primer sequences were designed according to mouse, rat or human cDNAs and/or RefSeq nucleotide sequences of reference, as indicated.