Fig. 7.

Database-independent sequence reconstruction of N3ICD protein (Q61982), using ProAlanase and trypsin. De novo sequencing plot showing a 89% coverage of N3ICD protein (Q61982) by unique ProAlanase and tryptic de novo peptides, obtained from in-gel digested N3ICD immunoprecipitates. The two biological replicates of ProAlanase- and trypsin-digested N3ICD were merged in as single data search, by protease. PEAKS X+ Studio was used to generate ProAlanase and tryptic de novo peptide sequences. The generated peptides were _filtered using a 90% De Novo score threshold and duplicates were removed. Sequence alignment was performed using PepExplorer within PatternLab for Proteomics.