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. 2020 Nov 5;5(21):e141183. doi: 10.1172/jci.insight.141183

Figure 3. Glycolysis and pentose phosphate pathway metabolites changed in Ndufs3-KO brains.

Figure 3

Cortices from 2.5-month-old Ndufs3 nKO male mice, compared with those from control littermates, were used for the analysis (n = 6/group). (A) Euclidean Heatmap of top 25 metabolite changes showing a clear clustering of KO and controls. (B) Schematics of glycolysis and pentose phosphate pathways, with increased metabolites in orange. (C) Heatmap of average KO and control values for glycolysis intermediates. Both P and q values are shown. (D) Heatmap of average KO and control values for pentose phosphate pathway intermediates. Both P and q values are shown. (E) Hexokinase enzyme activity in brain homogenates of 4-month-old male Ndufs3 nKO and control mice (n = 7–8/group). Groups were compared using Welch’s 2 sample t test, and q values were determined from the significant hits with P < 0.05. **P < 0.01.