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. 2020 Nov 19;5(22):e139910. doi: 10.1172/jci.insight.139910

Figure 7. Isolated LV skinned CMs from CM GC-B–KO mice with TAC showed enhanced stiffness, which was reversed by addition of recombinant PKGI.

Figure 7

(A) Stretch protocol used in these experiments (sarcomere lengths 1.8–2.4 μm). (B–D) Single skinned CMs were prepared from sham control (CTR) and CM GC-B–KO (KO) mice (B) and from both genotypes after 3 days (C) or 14 days (D) of TAC for recordings of the force response to stepwise cell stretching in relaxing buffer. Top panels in C and D: examples of original recordings. (Bottom) In addition passive force (Fpassive) in relation to sarcomere length was recorded for control and GC-B–KO CMs from mice with TAC without or with pretreatment with recombinant PKGI. Data are shown as means ± SEM. n = 16–24 CMs from 4–5 hearts per group. *P < 0.05 KO versus CTR (1-tailed Student’s t test); #P < 0.05 KO without and with PKGI (2-tailed Student’s t test); †P < 0.05 CTR without and with PKGI (2-tailed Student’s t test).