Figure 6. aPDT with 1D9-700DX in a human postmortem infection model.

Snap shots of the positioning of nitrocellulose membranes with spotted 1D9-700DX–labeled S. aureus on the lateral side of the proximal tibia of a human postmortem leg, followed by irradiation with red light at a radiant exposure of 30 J.cm^–2, or no light exposure. Numbers of surviving bacteria (Log₁₀[CFU/mL]) on implanted or nonimplanted nitrocellulose membranes upon treatment with red light at a radiant exposure of 30 J.cm^–2 (P⁺L⁺) or no light exposure (P^–L^–). Overnight-cultures of S. aureus (clinical isolate) or S. epidermidis ATCC 35984 (negative control) were incubated with 3.3 μM of 1D9-700DX and spotted on nitrocellulose membranes, which were either kept outside (nonimplanted) or implanted in the human postmortem leg. Bacterial survival was assessed by replica plating of the membranes onto BA plates and overnight incubation at 37°C (n = 1 postmortem leg).