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. 2020 Nov 5;5(21):e137474. doi: 10.1172/jci.insight.137474

Figure 1. Effects of ACA on body weight dynamics, muscle mass, and body temperature.

Figure 1

(A and B) Body weight was assessed monthly from the initiation of treatment at 4 months until 25 months of age (A), and data were used to calculate weight change across different time points (B). n = 16–19 per sex and treatment group at the start of the study, with 14–16 mice per group remaining alive at 24 months. (C) At 25 months, animals were dissected and quadriceps weight was higher in male mice treated with ACA compared with controls. (D) In a separate cohort of mice treated with ACA from 4 months, body temperature was assessed at 22 months of age and compared with untreated animals that were of the same age, in addition to younger 6 month old animals. P values in C were calculated with an ANCOVA, including body weight as a covariate. In D and E, P values are calculated from a Dunnett’s multiple comparisons test, comparing young animals and old ACA-treated animals with old controls in separate comparisons, after conducting an ANOVA. Each dot represents a data point from an individual animal.