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. 2020 Nov 5;5(21):e140040. doi: 10.1172/jci.insight.140040

Figure 4. Modest neurodegeneration detected by Fluoro-Jade staining during early EAE.

Figure 4

(A and C) No evidence of Fluoro-Jade+ staining was seen in brain tissue sections from unimmunized control mice; representative images of cerebellum (A) and brainstem (C). (B and D) Fluoro-Jade+ foci (indicated with arrows) were observed in cerebellum (B) and brainstem (D) of mice at day 11 p.i. (Fluoro-Jade, green; DAPI, blue). The Fluoro-Jade+ foci consistently accompanied inflammatory foci (B and D). (E) Quantification of fluorescence intensity of the tissue section showed an increasing accumulation of Fluoro-Jade staining by day 11 p.i. (arbitrary units) (n = 15).