Figure 3. Cardiac valve anatomy and surface areas in neonatal mice.

(A) Representative images of heart cross sections stained with H&E. Dotted boxed areas indicate the aortic, pulmonary, mitral (blue) and tricuspid (black) valves of WT, Kras^T58I/+, and CMV-Cre Kras^LSL-P34R/+ animals. Scale bars: 1 mm. (B) Quantitation of heart valve areas in WT (dark blue circles), Kras^T58I/+ (red squares), and CMV-Cre Kras^LSL-P34R/+ (light blue triangles) neonatal mice. Statistically significant differences between individual genotypes are shown and were evaluated by ordinary 1-way ANOVA with Tukey’s multiple comparisons test. (Note: the P value for the pulmonary valve areas of Kras^T58I/+ animals is P = 0.0587.)