Figure 3. Sample selection for proteomic analysis of serum Pfs25 Abs.

(A) Serum samples presenting high functional activity (transmission-reducing activity, TRA >90%) are highlighted in gray. Subject ID is listed by increasing level of functional activity, or %TRA. %TRA is calculated as the percentage reduction in the number of oocysts counted on mosquito midguts 8 days after feeding on cultured parasites mixed with individual postvaccination serum and compared with mosquitoes fed with human naive sera. (B) Correlation between anti-Pfs25 Ab titers and functional activity (TRA) in sera of vaccinated subjects. (C) Coomassie gel containing the digestion products of Pfs25-IG purified from plasma. The region corresponding to F(ab′)₂ was excised from the gel and analyzed by tandem mass spectrometry (MS/MS). (D) Spearman’s correlation between the number of peptide matches in the IMGT database and those in the IGH CDR3 data set was statistically significant.