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. 2020 Nov 5;5(21):e140627. doi: 10.1172/jci.insight.140627

Figure 3. VINAS knockdown decreases inflammation in SMCs and BMDMs.

Figure 3

VINAS knockdown decreases mRNA levels of VCAM-1 (A, n = 4), ICAM-1 (B, n = 6), and MCP-1 (C, n = 4) in MOVAS smooth muscle cells (SMCs) stimulated with 5 ng/mL TNF-α. VINAS knockdown decreases protein expression of VCAM-1 (D, n = 4), ICAM-1 (E, n = 4), MCP-1 (F, n = 3), and IL-1β (G, n = 4) in MOVAS smooth muscle cells stimulated with 20 ng/mL TNF-α. VINAS knockdown decreases the protein expression of TNF-α (H), COX-2 (I), and IL-1β (J) in bone marrow–derived macrophages (BMDMs) stimulated with 50 ng/mL LPS (n = 3). Data represent the mean ± SD. Statistical differences were calculated using unpaired 2-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001.