Figure 7.

LAM-like tumor growth in immunocompetent mice. We tested the characteristics of LAM-like cells hosted by immunocompetent mice by subcutaneous and i.v. challenge of C57BL/6 mice with LAM-like cells. (A) Subcutaneous tumor growth was observed 2 months after subcutaneous challenge. Representative staining is shown for (B) gp100, (C) pS6, and (D) Tsc2, revealing the LAM-like identity of tumor cells. Immune infiltration was observed by detecting (E) CD3 and PD-1 co-localization, (F) the presence of F4/80⁺ macrophages, and (G) FoxP3 and CD3 co-localization indicative of regulatory T cells in an LAM-like subcutaneous tumor. Scale bars: 100 μm.