Skip to main content
. 2020 Nov 16;9:e59157. doi: 10.7554/eLife.59157

Figure 6. Conserved VDML activity during enhancer swaps in Phlebobranchia (A).

Representative examples of X-gal stained embryos at tailbud stages following C. intestinalis (B), P. mammillata (C), P. fumigata (D), or A. mentula (E) embryo electroporation with C. intestinalis or P. mammillata CRMs. All shown CRMs for the genes Msx (i), Ascl.b (ii), Klf1/2/4 (iii), Nkxtun3 (iv), Tox (v), and Dlx.c (vi) are active in VDML in their species of origin. The name of the electroporated CRM is indicated on each picture. Details for each experiment can be found in Figure 6—figure supplement 1 and Figure 6—figure supplement 2. Embryos are shown in lateral view with dorsal to the top and anterior to the left. Scale bar: 50 μm.

Figure 6.

Figure 6—figure supplement 1. Conserved VDML activity during enhancer swaps between C. intestinalis and P. mammillata.

Figure 6—figure supplement 1.

Schematic representation of the various constructs and their activity at tailbud stages in VDML (blue) (n indicates the total number of embryos examined, N indicates the number of independent experiments).
Figure 6—figure supplement 2. C. intestinalis and P. mammillata midline CRMs are active in VDML of P. fumigata and A. mentula embryos.

Figure 6—figure supplement 2.

Schematic representation of the various constructs and their activity at tailbud stages in VDML (blue) (n indicates the total number of embryos examined, N indicates the number of independent experiments).