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Operation of the liver-on-a-chip. Step 1: Deposition of HUVEC, LX-2, and HepG2 cells simultaneously in the central microchannel by laminar flow; Step 2: Perfusion of U937 cells through the “artificial blood” channel. Step 3: Incubation for 2 h for attachment of U937 cells. Step 4: Loading of hepatoprotectants. Step 5: Loading of APAP.
